Screen Quest Calbryte 590 免丙磺舒和免洗鈣檢測(cè)試劑盒是美國(guó)AAT Bioquest研發(fā)的用于檢測(cè)鈣離子的試劑盒鈣通量測(cè)定是用于篩選G蛋白偶聯(lián)受體（GPCR）的藥品發(fā)現(xiàn)中的優(yōu)選方法。 Screen Quest Calbryte-590不含Probenecid和Wash-Free的鈣測(cè)定試劑盒提供強(qiáng)大的均相熒光測(cè)定，用于檢測(cè)細(xì)胞內(nèi)鈣動(dòng)員。表達(dá)感興趣的GPCR的細(xì)胞通過鈣預(yù)先加載我們專有的Calbryte -590NW，其可以穿過細(xì)胞膜。 Calbryte -590 NW是適合HTS篩查的鈣指示劑。一旦進(jìn)入細(xì)胞內(nèi)，Calbryte -590NW的親脂性阻斷基團(tuán)被非特異性細(xì)胞酯酶切割，導(dǎo)致帶電荷的熒光染料停留在細(xì)胞內(nèi)，并且在與鈣結(jié)合后其熒光大大增強(qiáng)。當(dāng)用篩選化合物刺激細(xì)胞時(shí)，該受體表明細(xì)胞內(nèi)鈣的釋放，這極大地增加了Calbryte -590NW的熒光。其優(yōu)異的細(xì)胞保留特性，高靈敏度和100-250倍的熒光增加（當(dāng)它與鈣形成復(fù)合物時(shí)）使Calbryte -590NW成為測(cè)量細(xì)胞鈣的理想指標(biāo).Calbryte -590NW是鈣染料不需要丙磺舒以獲得更好的細(xì)胞保留。這款Screen Quest Calbryte-590不含Probenecid和Wash-Free的鈣測(cè)定試劑盒提供了優(yōu)化的檢測(cè)方法，用于監(jiān)測(cè)G蛋白偶聯(lián)受體（GPCR）和鈣通道與脆弱或困難的細(xì)胞系。該測(cè)定可以以方便的96孔或384孔微量滴定板形式進(jìn)行，并且易于適應(yīng)自動(dòng)化。百螢生物是AAT Bioquest 的中國(guó)代理商，為您提供的鈣檢測(cè)試劑盒

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